PKA induced phosphorylation of TG2 at Ser
facilitates downregulation of PTEN mRNA. Histogram showing relative quantification (RQ) of PTEN mRNA expression levels as determined by real-time PCR in response to PKA activation by db-cAMP in MEFtg2-/- and breast cancer cells in comparison to empty vector control group. The levels of mRNA were normalized against GAPDH mRNA. Cell transfection and treatments were performed as described in the figure legends 1 and 2. Data are represented as mean ± SEM, n = 4. **P < 0.01 (TG2 vs. vector control).