Secondary structure assignment and homology remodeling. (A) Chemical shift based secondary structure assignment in GIG47 and 1TOG. The overall secondary structure distribution determined by Cα chemical shifts in two proteins is very similar except for the additional solubility tagging regions at the protein termini of GIG47. (B) The ribbon diagrams for GIG47 and 1TOG. The structure of GIG47 was obtained by NMR chemical shifts and homology modeling using 1TOG as a template. The two structures are highly superimposable except for the additional solubility tag regions at the protein termini of GIG47.