BLU expression in CNE-2 cells following adenovirus-mediated gene transfer. (A–F) CNE 2 cells plated in six-well culture plates were challenged with 0, 10, 50, 100, 200, or 400 PFU viral particles. (G-I) Cells were grown on cover slips, fixed and viewed with a confocal microscope at a resolution of × 100. The cells were identically manipulated, stained with 3 M PI, and viewed with confocal microscope at a resolution of × 400 for EGFP. The overlapping signals were analyzed on merged figures. The arrows indicate cytoplasmic localization of BLU protein.