Induction of cell death events increases hornerin expression and fragmentation. (A) Quantitative real time PCR analysis of hornerin transcript abundance in MCF10AI cells treated overnight with increasing concentrations of H2O2. Data are the mean +/− SEM of three independent experiments; * P < 0.05. (B) Representative western blot of hornerin fragments, caspase 3 and cleaved caspase 3 in MCF10AI cells treated with the indicated concentrations of H2O2. α-Tubulin was used as a loading control.