Hornerin expression in an in vitro model of breast cancer progression. (A) Quantitative real time PCR analysis of hornerin transcript abundance in proliferating MCF10A cell line series. Quantified data were normalized to the housekeeping gene GAPDH. (B) Western blot analysis of hornerin expression in proliferating MCF10A cell line series; α-tubulin was used as a loading control. (C) Data represent mean +/− SD of three independent experiments. Quantified values were expressed relative to corresponding abundance of tubulin protein expression *P < 0.05.