Figure 2From: Hornerin, an S100 family protein, is functional in breast cells and aberrantly expressed in breast cancer Hornerin expression throughout the stages of mammary development. (A) Representative images of mammary glands isolated from the indicated stages of development that were subjected to immunohistochemical analysis using a hornerin-specific antibody or corresponding negative control. (B) Data represent the mean +/− SD of a minimum of three glands quantitated using Image J64. *P < 0.05 compared to nulliparous and pregnant glands. (C) Data represent the mean +/− SD of number of hornerin positive macrophages counted per 40x field; a minimum of three glands was counted at each developmental stage and three fields were counted per slide. * *P < 0.05 compared to nulliparous glands. (D) Representative images of immune cells in mammary glands that were subjected to immunohistochemical analysis using a hornerin-specific antibody and the corresponding PCR analysis of hornerin in human monocytes/macrophages isolated from peripheral blood; cells were treated +/− 20 ng/ml of LPS/IFNγ for five days. N = nulliparous week 5, Pg = pregnancy day 9, L D1 = lactation day 1, L D15 = lactation day 15, I D1 = involution day 1, I D7 = involution day 7. Yellow bar = 200 μm, Red bar = 50 μm.Back to article page