Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

Table 1 The primers and TaqMan probes of IGF, ER genes used for RT-PCR

Genes (Accession No.)	Primers and probe	size	Annealing temperature
IGF-1 (NM_000618)	Forward: 5-AGCTGTGATCTAAGGAGGCTGG-3’	143 bp	57 °C
	Reverse: 5’-GCACTCCCTCTACTTGCGTTCTT-3’
	Probe: 5’-(FAM)-TCAGCTCGCTCTGTCCGTGCCC-3’(TAMRA)
IGF-1R (NM_000875)	Forward: 5’-CTTGTACATTCGCACCAATGCT-3’	83 bp	59 °C
	Reverse: 5’-CGATTAACTGAGAAGAGGAGTTCGA-3’
	Probe: 5’-CTTCCATTCCCTTGGACGTTCTTTCAGC-3’
IGF-2 (NM_000612)	Forward: 5’-AGGAGCTCGAGGCGTTCA-3’	65 bp	55 °C
	Reverse: 5’-GTCTTGGGTGGGTAGAGCATC-3’
	Probe: 5’-AGGCCAAACGTCACCGTCCCC-3’
IGF-2R (NM_000876)	Forward: 5’-GCGGCACACCCTATAACAATG-3’	74 bp	59 °C
	Reverse: 5’-CGCGTCTCGATCACAGAGAA-3’
	Probe: 5’-AAGACACACACCGAGAGCTACGCTCATCA-3’
IGFBP-3 (NM_000596)	Forward: 5’-CAGGAGACATCAGGAGAAGAAATTT-3’	117 bp	59 °C
	Reverse: 5’-TCCCGCCTCTCCATCCAT-3’
	Probe: 5’-TTACCTGCCAAACTGCAACAAGAATGGATT-3’
ERα (NM_000125)	Forward: 5’-AGAGGGCATGGTGGAGATCTT-3’	83 bp	59 °C
	Reverse: 5’-CAAACTCCTCTCCCTGCAGATT-3’
	Probe: 5’-(FAM)TGATGACACTGCCACTCCTGAGGCA-3’(TAMRA)
ERβ (NM_001437)	Forward: 5’-GACCACAAGCCCAAATGTGTT-3’	69 bp	59 °C
	Reverse: 5’-AACTGGCGATGGACCACTAAA-3’
	Probe: 5’-(FAM)TGGCCAACACCTGGGCACCTTT-3’(TAMRA)
GAPDH (NM_002046)	Forward: 5’-CCAGGTGGTCTCCTCTGACTT-3’	130 bp	59 °C
	Reverse: 5’-GTTGCTGTAGCCAAATTCGTTGT-3’
	Probe: 5’-(FAM)AACAGCGACACCCACTCCTCCACC-3’(TAMRA)

ISSN: 1471-2407