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Figure 9 | BMC Cancer

Figure 9

From: TGF-β1 modulates the homeostasis between MMPs and MMP inhibitors through p38 MAPK and ERK1/2 in highly invasive breast cancer cells

Figure 9

In vitro migration (A) and invasion (B) capacities of the MDA-MB-231 cell line upon treatment with TGF-β1 and inhibitors of ERK1/2, p38 MAPK and MMPs. MDA-MB-231 cells were pre-treated for 1 h with 20 μM of either PD98059 or SB203680 (ERK1/2 and p38 MAPK pharmacological inhibitor, respectively) or with 40 μM of GM6001 (a broad-spectrum MMPs inhibitor). After the inhibition treatment these cells were stimulated with 10 ng/mL TGF-β1 and allowed to migrate through uncoated transwells for 8 h (A) or invade through matrigel-coated transwells for 24 h (B). The number of cells at the bottom of the transwell filters was counted at the end of each assay. Results are presented as means ± standard errors from three independent experiments, performed in duplicate, all versus control (cells treated with DMSO, vehicle).

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