Cyclin A1 shows age-related expression in benign tonsils, HPV16-dependent overexpression in HNSCC and predicts lower recurrence rate in HNSCC independently of HPV16

Table 6 Primers and probes used for p53 mutation analysis

Exon	Primer		Sequences (5’ → 3’)	Annealing Temperature (°C)
2-4	A1	forward	TCAGACACTGGCATGGTGTTG	64
	A1	reverse	AGGGTGAAGAGGAATCCCAAAG	64
	A2	forward	GTGAGTGGATCCATTGGAAG	64
	A2	reverse	GTGAAAAGAGCAGTCAGAGG	64
5/6	B	forward	TAGTGGGTTGCAGGAGGTGC	62
5/6	B	reverse	GAGGCCCTTAGCCTCTGTAAGC	62
7	C	forward	CTGCTTGCCACAGGTCTCC	62
7	C	reverse	CGGGGATGTGATGAGAGGTGG	62
8/9	D	forward	AAGGACAAGGGTGGTTGGGAG	62
8/9	D	reverse	CAACCAGGAGCCATTGTCTTTG	62

ISSN: 1471-2407