Cyclin A1 shows age-related expression in benign tonsils, HPV16-dependent overexpression in HNSCC and predicts lower recurrence rate in HNSCC independently of HPV16

Table 5 Primers and probes used for HPV16-Real-Time PCR

Gene		Sequence (5’ → 3’)
HPV16 E6	Primer1, forward	CTGCAATGTTTCAGGACCCA
	Primer2, reverse	TCATGTATAGTTGTTTGCAGCTCTGT
	Probe	FAM-AGGAGCGACCCAGAAAGTTACCACAGTT-TAMRA
HPV16 E7	Primer1, forward	AAGTGTGACTCTACGCTTCGGTT
	Primer2, reverse	GCCCATTAACAGGTCTTCCAAA
	Probe	FAM-TGCGTACAAAGCACACACGTAGACATTCGTA-TAMRA
HBB	Primer1, forward	GTGAAGGCTCATGGCAAGAAAG
	Primer2, reverse	CAGCTCACTCAGTGTGGCAAAG
	Probe	FAM-ATGGCCTGGCTCACCTGGACAACC-TAMRA

PCR: Polymerase chain reaction; HPV16: Human Papillomavirus 16; E6, E7: Oncogenes of HPV16; HBB: Human beta-globin.

ISSN: 1471-2407