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Table 4 Correlation of Cyclin A1 expression in immunohistochemistry with clinicopathological characteristics in HNSCC

From: Cyclin A1 shows age-related expression in benign tonsils, HPV16-dependent overexpression in HNSCC and predicts lower recurrence rate in HNSCC independently of HPV16

Clinicopathological parameter N Performed Statistic Subgroups P
Age [y] 81 Spearman Rank Order σ = −0.070 0.532
Sex 81 Mann–Whitney Rank Sum male vs. female 0.964
Primary site     
Tonsil 33 Fisher exact CCNA1 <4 vs. ≥4 0.623
Base of the tongue 28 Fisher exact CCNA1 <4 vs. ≥4 0.447
Anterior two-thirds of the tongue 9 Fisher exact CCNA1 <4 vs. ≥4 0.268
Floor of the mouth 2 Fisher exact CCNA1 <4 vs. ≥4 1.000
Hypopharynx 1 Fisher exact CCNA1 <4 vs. ≥4 1.000
Soft palate 2 Fisher exact CCNA1 <4 vs. ≥4 1.000
Supraglottic 3 Fisher exact CCNA1 <4 vs. ≥4 0.551
Unknown (CUP) 3 Fisher exact CCNA1 <4 vs. ≥4 0.208
T 72 Fisher exact T1/2 vs. T3/4 0.591
N 73 Fisher exact N0/1 vs. N2/3 0.312
M 75 Fisher exact M0 vs. M1 0.094
G 62 Fisher exact G1/2 vs. G3/4 0.404
UICC stage 71 Fisher exact stage1/2 vs. stage3/4 1.000
Recurrence rate 64 Mann–Whitney Rank Sum Recurrence vs. no recurrence univariate 0.002
multivariate 0.013
Secondary tumor 78 Mann–Whitney Rank Sum sec. tumor vs no sec. tumor 0.516
Nicotine abuse 79 Mann–Whitney Rank Sum nicotine vs. no nicotine 0.358
Alcohol abuse 79 Mann–Whitney Rank Sum alcohol vs. no alcohol 0.743
Progression free survival 81 Kaplan-Meier CCNA1 <4 vs. ≥4 0.363
Overall survival 81 Kaplan-Meier CCNA1 <4 vs. ≥4 0.354
HPV16 DNA status 81 Mann–Whitney Rank Sum HPV16+ vs. HPV16- 0.001
E6 quantitative 37 Spearman Rank Order σ = 0.229 0.172
E7 quantitative 37 Spearman Rank Order σ = 0.250 0.134
P16 Expression 79 Spearman Rank Order σ = 0.376 0.0007
P53 Expression 80 Spearman Rank Order σ = −0.086 0.448
P53 Mutation 41 Mann–Whitney Rank Sum P53 mutated vs. wild-type 0.920
Cyclin A1 Methylation 44 Mann–Whitney Rank Sum methylated vs. non-methylated 0.882
  1. N: number of available samples; CUP: Carcinoma of unknown primary; Age in years [y]; stage: UICC-stage; T: Tumor size: T1 (< 2 cm), T2 (2-4 cm), T3 (> 4 cm), T4 (Tumor infiltrates adjacent structures); N: status of regional lymph node metastasis: N0 (no metastasis), N1 (single ipsilateral lymph node metastasis, size < 3 cm), N2a (single ipsilateral lymph node metastasis, size 3-6 cm), N2b (multiple ipsilateral lymph node metastases, size 3-6 cm), N2c (multiple ipsi- and contralateral lymph node metastases, size 3-6 cm), N3 (lymph node metastasis, size > 6 cm); E6/E7 quantitative: results of quantitative HPV16 Real-Time PCR; CCNA1: Cyclin A1; CCNA1 <4/≥4: immunohistochemical expression of Cyclin A1 less than 20% or at least 20%.