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Table 1 Results of HPV16 RT-PCR, immunohistochemistry of p16 , Cyclin A1 , and p53 and p53 mutation analysis

From: Cyclin A1 shows age-related expression in benign tonsils, HPV16-dependent overexpression in HNSCC and predicts lower recurrence rate in HNSCC independently of HPV16

  HNSCC (N = 81) Controls/Tonsils (N = 74) P
Age (mean ± sd) 62.78 ± 11.24 27.19 ± 19.26 <0.001
Sex Male 53 40 0.200
Female 28 34
Cyclin A1 overexpression (abs./%) Total 24/29.6 4/5.4 <0.001
Category 0 (negative) 0/0.0 2/2.7  
Category 1 (few positive cells) 4/4.9 9/12.2
Category 2 (< 10%) 25/30.9 34/45.9
Category 3 (10-20%) 28/34.6 25/33.8
Category 4 (20-30%) 14/17.3 4/5.4
Category 5 (30-40%) 5/6.2 0/0.0
Category 6 (40-50%) 3/3.7 0/0.0
Category 7 (50-60%) 1/1.2 0/0.0
Category 8 (60-70%) 1/1.2 0/0.0
Category 9 (70-80%) 0/0.0 0/0.0
Category 10 (80-90%) 0/0.0 0/0.0
Category 11 (90-100%) 0/0.0 0/0.0
HPV16 positive (abs./%) Total 37/45.7 0/0.0  
P16 positive 29 out of 37/78.4  
Cyclin A1 positive 18 out of 37/48.6  
HPV16 negative (abs./%) Total 44/54.3 74/100.0  
P16 positive 4 out of 44/9.1 0 out of 74/0.0
Cyclin A1 positive 6 out of 44/13.6 4 out of 74/5.4
P16 overexpression (abs./%) Total 33 out of 79/41.8 0/0.0  
HPV16 negative 4 out of 33/12.1  
P53 overexpression (abs./%) Total 33 out of 80/41.3 0/0.0  
HPV16 positive 4 out of 33/12.1  
P53 mutation (abs./%) Total 19 out of 41/46.3 0 out of 30/0.0  
HPV16 positive 4 out of 19/21.1  
P53 positive 9 out of 19/47.4  
  1. Cut off for overexpression in immunohistochemistry: CDKN2A/p16 positive: ≥20% (category ≥4); Cyclin A1 positive: ≥20% (category ≥4); p53 positive: ≥10% (category ≥3).