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Table 1 Results of HPV16 RT-PCR, immunohistochemistry of p16 , Cyclin A1 , and p53 and p53 mutation analysis

From: Cyclin A1 shows age-related expression in benign tonsils, HPV16-dependent overexpression in HNSCC and predicts lower recurrence rate in HNSCC independently of HPV16

 

HNSCC (N = 81)

Controls/Tonsils (N = 74)

P

Age (mean ± sd)

62.78 ± 11.24

27.19 ± 19.26

<0.001

Sex

Male

53

40

0.200

Female

28

34

Cyclin A1 overexpression (abs./%)

Total

24/29.6

4/5.4

<0.001

Category 0 (negative)

0/0.0

2/2.7

 

Category 1 (few positive cells)

4/4.9

9/12.2

Category 2 (< 10%)

25/30.9

34/45.9

Category 3 (10-20%)

28/34.6

25/33.8

Category 4 (20-30%)

14/17.3

4/5.4

Category 5 (30-40%)

5/6.2

0/0.0

Category 6 (40-50%)

3/3.7

0/0.0

Category 7 (50-60%)

1/1.2

0/0.0

Category 8 (60-70%)

1/1.2

0/0.0

Category 9 (70-80%)

0/0.0

0/0.0

Category 10 (80-90%)

0/0.0

0/0.0

Category 11 (90-100%)

0/0.0

0/0.0

HPV16 positive (abs./%)

Total

37/45.7

0/0.0

 

P16 positive

29 out of 37/78.4

 

Cyclin A1 positive

18 out of 37/48.6

 

HPV16 negative (abs./%)

Total

44/54.3

74/100.0

 

P16 positive

4 out of 44/9.1

0 out of 74/0.0

Cyclin A1 positive

6 out of 44/13.6

4 out of 74/5.4

P16 overexpression (abs./%)

Total

33 out of 79/41.8

0/0.0

 

HPV16 negative

4 out of 33/12.1

 

P53 overexpression (abs./%)

Total

33 out of 80/41.3

0/0.0

 

HPV16 positive

4 out of 33/12.1

 

P53 mutation (abs./%)

Total

19 out of 41/46.3

0 out of 30/0.0

 

HPV16 positive

4 out of 19/21.1

 

P53 positive

9 out of 19/47.4

 
  1. Cut off for overexpression in immunohistochemistry: CDKN2A/p16 positive: ≥20% (category ≥4); Cyclin A1 positive: ≥20% (category ≥4); p53 positive: ≥10% (category ≥3).