Figure 4

Stabilisation of PPARGC1A mRNA in the lymphoblastoid cell lines (LCLs) of individuals from Family B after caffeine (7.5mM) treatment measured by semi-quantitative real-time RT-PCR. PPARGC1A mRNA is stabilised in family members affected by breast cancer and not in unaffected family members, with the exception of Person 4. Each sample has been normalised to the housekeeping gene, GAPDH, and calibrated to the lowest expressing untreated sample. Standard error bars represent standard error from the mean of four technical replicates for each PCR reaction.