Figure 4
From: Microtubule S-glutathionylation as a potential approach for antimitotic agents
2-AAPA induces microtubule depolymerization in UACC-62 cells. UACC-62 cells were treated with the indicated concentrations of 2-AAPA for 12 h (a), or with 50 μM 2-AAPA for indicated time periods (b), followed by fixation, permeabilization and indirect immunofluorescent analysis with an anti-α-tubulin-FITC. Nuclei were stained with DAPI. Paclitaxel and vinblastine were employed as positive controls for microtubule stabilization and microtubule depolymerization respectively. The data are derived from one of the three independent experiments.