Figure 1
From: The role of TGFBI in mesothelioma and breast cancer: association with tumor suppression
Expression of TGFBI mRNA and protein in TGFBI-transfected, empty vector control, and parental tumor cells. (A) Cells were transfected with pRc/CMV2-TGFBI and pRc/CMV2-empty vector. The expression of TGFBI mRNA was analyzed by quantitative real-time RT-PCR. The value was used to plot TGFBI expression using 2▽▽Ct. Each value is expressed in triplicate (error bars; mean ± SD). TGFBI-transfected mesothelioma cells (T2804, T2806, and T2807), pRc/CMV2- vector control cells (V2801), and parental mesothelioma cells NCI-H28. Met-5A is an immortalized human mesothelial epithelial cell line. (B) TGFBI-transfected breast cancer cells (T23108, T23109 and T23113), pRc/CMV2- vector control cell (V23101), and parental breast tumor cell MDA-MB-231. MCF-10 F is a spontaneously derived immortalized human breast epithelial cell line. Expression of TGFBI protein in (C) mesothelioma cells and (D) breast tumor cells was measured with Western blotting (upper) and immunohistochemical staining (lower, ×400).