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Figure 3 | BMC Cancer

Figure 3

From: Effect of inhibition of the Ubiquitin-Proteasome System and Hsp90 on growth and survival of Rhabdomyosarcoma cells in vitro

Figure 3

Analysis of apoptosis induction in RH30 and RD Rhabdomyosarcoma cells. To assess the role of autophagy in drug-treated RH30 and RD cells, apoptosis was measured by flow- cytometry after staining treated and untreated cells (Bortezomib 7,5nM; 17-DMAG 50nM; Bortezomib/17-DMAG) with Annexin-V-FITC/Propidium Iodide (AV/PI) in the presence or absence of non-toxic concentrations of rapamycin (5 ng/mL). Non-apoptotic (healthy, AV/PI), apoptotic (AV+/PI and AV+/PI+) and necrotic cells (AV/PI+) were identified, and their relative percentages reported in the corresponding quadrants. The relative amount of each fraction is graphed aside.

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