Cyp40, but not FKBP51 or FKBP52, promotes ALK+ ALCL viability. (A) The viability of Karpas 299 (upper) or SUP-M2 (lower) cells transfected with the indicated pooled siRNAs was measured using the MTS assay (left). Western blots (right) demonstrate the silencing efficiency of the targeted proteins. * indicates a non-specific band in the anti-FKBP51 blot. (B) Viability of Karpas 299 (upper left) or SUP-M2 (lower left) cells transfected with the indicated pooled siRNAs. Combined siRNA consists of siRNAs to target the three immunophilin co-chaperones, Cyp40, FKBP51 and FKBP52. Western blots (right) demonstrate the level of silencing of the indicated proteins. Quantification represents the mean and standard deviation of four independent experiments. p values comparing cells transfected with the indicated siRNA to those transfected with control siRNA were obtained using paired, one-tailed t-tests. p values comparing cells transfected with Cyp40 or combined siRNA were obtained using unpaired, one-tailed t-tests. N.S. indicates a p value >0.05.