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Figure 5 | BMC Cancer

Figure 5

From: MicroRNA-182 downregulates metastasis suppressor 1 and contributes to metastasis of hepatocellular carcinoma

Figure 5

miR-182 directly targets human MTSS1. A: 3’-UTR region of MTSS1 mRNA is partially complementary to miR-182. Target-Scan and Pictar analysis revealed two miR-182 binding sites in MTSS1 UTR sequence. B: Effect of miR-182 on the luciferase activity of Luc-MTSS1-3’UTR and Luc- MTSS1-3’UTR mutation. The assay was done in HUH-1 cells as described in Materials and Methods. Renilla and firefly luciferase activities were measured with the Dual-Luciferase Reporter system (Promega) 24 h after transfection. Firefly luciferase activity was normalized to Renilla luciferase expression for each sample. Each experiment was performed in triplicate. Data are shown as mean ± s.d. *p < 0.01.

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