Figure 2
From: Transforming growth factor-β suppresses metastasis in a subset of human colon carcinoma cells
Enhanced cell survival capability of FETα-DN cells results in metastasis. Primary tumors established in animals orthotopically implanted with FETα and FETα-DN tumors were processed for (A-B) KI-67 staining and were analyzed to evaluate the proliferation rate. Primary tumors established in animals orthotopically implanted with FETα and FETα-DN tumors were processed for (C-D) TUNEL staining and were analyzed to evaluate the apoptotic rate. Both KI-67 and TUNEL images were captured at 200x magnification. Image J software was employed to quantify positive staining cells and the total number of cells. Statistical significance was determined using two tailed student’s t test with p value less than 0.05.