Figure 3

Effect of mutation and methylation of site CGCG (−695~-692) on MYCT1 promoter activity in Hep-2 cells. A. Schematic representation of the plasmid constructs P852-mutB created by site-directed mutagenesis. The arrows indicate the primers used for constructing the mutant construct. B. Luciferase activities in Hep-2 cells of the presence or absence of 5-aza transfected with wild-type and mutant vectors of MYCT1.