Figure 4From: Penetration of anticancer drugs through tumour tissue as a function of cellular packing density and interstitial fluid pressure and its modification by bortezomib Intensity of doxorubicin fluorescence (I) plotted against distance from the nearest blood vessel (μm) is shown for HCT-8Ea and Ra (A) and HCT-8E11/1R1 (C) xenografts. Mean values are shown for ~160 areas of interest from 20 tumours in 10 mice. Upper curves represent drug penetration through HCT-8R tumors, middle curves represent pre-treatment with bortezomib, while lower curves represent controls. Growth curves for HCT-8Ea/Ra (B) and HCT-8E11/1R1 (D) xenografts following single treatments with doxorubicin (8 mg/kg) with or without prior bortezomib (1 mg/kg). Greater growth delay was observed for the loosely packed HCT-81R1 tumour xenografts than their tightly packed HCT-8E11 counterparts (p = 0.0012) with similar results for HCT-8Ra and HCT-8Ea derived xenografts (p = 0.004). Bortezomib pre-treatment did not significantly enhance doxorubicin treatment efficacy in tumour xenografts derived from HCT-8Ea and E11 cell lines (p = 0.45 and p = 0.09 respectively). Data represent means and SEM for 10-12 mice.Back to article page