Table 1 Sequences of the PCR and pyrosequencing primers used in this study
Gene symbol | Primer sequences (5´-3´) * F: PCR forward; R: PCR reverse; S: pyrosequencing; bio: biotinylation | Amplicon length, bp | Number of CpGs quantified by pyrosequencing | Reference |
|---|---|---|---|---|
CDH1-F | TTTTTTTTGATTTTAGGTTTTAGTGAG | 421 | [29] | |
CDH1-R | bio-ACTCCAAAAACCCATAACTAACC | |||
CDH1-S | AGTTAGTTTAGATTTTAGTT | 9 | this study | |
DACT1-F | GTTTGGGAAGTGAAAGAAATTTAATT | 184 | [30] | |
DACT1-R | bio-CTAAAACCCCAACATCCTATTACAAT | |||
DACT1-S | AGATTGTGTTGTAATTTGGT | 5 | this study | |
DKK1-F | bio-GGGGTGAAGAGTGTTAAAGGTT | 326 | [31] | |
DKK1-R | AAACCATCATCTCAAAAAAACTCAA | |||
DKK1-S | CTACAAAAAACACAAAACTCTAC | 8 | this study | |
DKK2-F | bio-TTTTAGTAGTTGTGGGTGGAGATA | 456 | this study | |
DKK2-R | ATACTCCTTTTCAAAATTAACAAAC | |||
DKK2-S | CCTAACTCACAAAAAACAAC | 11 | ||
DKK3-F | GATTTTGTTGAGTTTAGTTTTTTTTGGT | 123 | [32] | |
DKK3-R | bio-CAAACCTCTCTCAACCCCTACCTA | |||
DKK3-S | TTTTTTGGTGGATGTG | 5 | this study | |
DKK4-F | bio-ATAGATTTGAAGGGATTTGTTGAAGTTT | 328 | [33] | |
DKK4-R | CAAAACCAACTCAACCCCAACAAAAC | |||
DKK4-S | CTAAACTAACAACTCAACAC | 2 | this study | |
SFRP1-F | TTTTTAAGGGGTGTTGAGT | 412 | [16] | |
SFRP1-R | CAAACTTCCAAAAACCTCC | |||
SFRP1-S | GGAGTTGATTGGTTG (Sanger sequencing) | this study | ||
SFRP2-F | ATGTTTGGTAATTTAGTAGAAATTT | 409 | this study | |
SFRP2-R | bio-CAACCAAAATTTTCTTAACCTTTTT | |||
SFRP2-S | GATTGGGGTAAAATAAGTT | 14 | ||
SFRP3-F | bio-GTGATTTAGGGGAGGAGATATTTTAGA | 542 | this study | |
SFRP3-R | TTCCAAAACAAAAACTTACACAAAA | |||
SFRP3-S | CAAAATAAAACAAAATACAAC | 4 | ||
SFRP4-F | bio-GTGTTTTGTGTGTTAGA | 220 | [16] | |
SFRP4-R | CCACTAAAATAAAAAAAAACATAACA | |||
SFRP4-S | TACCACCCTCATCTTTC | 2 | this study | |
SFRP5-F | GTAGGGAGTTTTGGGGAGAAA | 272 | [16] | |
SFRP5-R | bio-CCCAAATAAATAACAACCTAC | |||
SFRP5-S | GTTTTGGAGTTGGGGTTAG | 8 | this study | |
WIF1-F | bio-GAGTGATGTTTTAGGGGTTT | 414 | [34] | |
WIF1-R | CCTAAATACCAAAAAACCTAC | |||
WIF1-S | AAACTACATTCACAATAC | 7 | this study |