Imaging of a representative nulliparous homozygous MMTV-Luc2 female mouse.[Note: A-D images were obtained from the same representative female; please refer to Additional file 1 Figures S3 and S4 for additional examples] (A) Distribution of bioluminescent signal via whole body imaging. (B) Distribution of bioluminescent signal in whole-body autopsy. (C) Imaging of the isolated salivary glands. (D) Distribution of bioluminescent signal in isolated internal organs. B: brain, H: heart, Int: intestine, K: kidney, Lg: lungs, Lv: liver, S: spleen. [Please note that the ranges of the luminescence readout between panels A and D are 100-fold different, as shown by the colour bar scales]. (E) Tomographic DLIT™ 3D reconstitution of the luminescent signal distribution  at the wavelength of 660 nm from a representative homozygous MMTV-Luc2 female (ventral view). Reconstituted sources of luminescent light are shown as spheres (please note a superficial localisation of the light sources). Left-hand panels represent slices acquired by slice planes shown on the right hand panel: coronal (red), sagittal (blue) and transaxial (green).