Figure 4

Cell proliferation and migration in KATO III/mock, KATO III/wt-HB-EGF and KATO III/HB-EGF-mC cells. A) Cell proliferation assay. Mean of 3 independent clones; bars, SD;* P < 0.01, as compared with KATO III/mock; **P < 0.05. B) Transwell invasion assay was analyzed in each cell at 48 h after 200 nM TPA stimulation. Value of KATO-III/mock cells was arbitrarily defined as 1. Mean of 3 independent clones; bars, SD;* P < 0.01, as compared with KATO III/mock; **P < 0.05, as compared with KATO III/mock; ***P < 0.05. C) Wound healing assay. Confluent monolayers of each gastric cancer cells were mechanically wounded with a pipette tip, and photos were obtained at 0 h and 24 h after stimulation of 200 nM TPA (Original magnification: ×40). D) Quantification of wound healing assay in 3 independent clones. Migration rate of KATO-III/mock cells at 24 h after TPA stimulation was arbitrarily defined as 1. Mean of 3 independent clones; bars, SD* P < 0.01, as compared with KATO III/mock; **P < 0.01.