Figure 3

Gene expression profiling. (A) DAVID gene annotation enrichment analysis showing p80HT-regulated genes associated with various GO biological processes (FDR < 0.05). (B) Ingenuity pathway analysis of the same microarray data showing a network of genes relevant to human MM pathogenesis. Red indicates a significant increase in expression, green a significant decrease. Color intensity is proportional to fold change. Solid and dashed lines indicate a direct and indirect relationship, respectively. (C) qRT-PCR analysis of mRNA levels for CD27, cyclin D1, cyclin D2, IL-10, IL-15 and survivin in plasma cell tumor samples (n = 5) and p80HT B cells (n = 3) relative to those in wild-type B cells (n = 3, dashed line). Error bars represent the mean ± SEM of the measurements of two independent experiments with each being performed in triplicate.