Figure 1

Short-term cytotoxicity of cisplatin (CDDP) and paclitaxel (PTX) towards ovarian cancer cells is unchanged or enhanced by mifepristone (MF). (A) Number of ovarian cancer cells measured 4 days after exposure to 20 μM CDDP for 1 h, 100 nM PTX for 3 h, 10 μM MF for 4 days, the combination of CDDP plus PTX, or CDDP plus PTX followed by MF. (B) Hypo-diploid DNA content calculated for the same experimental groups depicted in (A). (C) A similar experiment was done in which all floating and adherent cells were pelleted, total DNA isolated, subjected to agarose electrophoresis, stained with SYBR Gold nuclei acid stain, and photographed with the Amersham Typhoon Fluorescence imaging system. A 100 base pair (bp) marker was run in parallel. (D) In a similar experiment as in (C), whole protein extracts were obtained and separated by electrophoresis, and immunoblots were probed with the indicated antibodies. The housekeeping gene GAPDH was used as protein loading control