Effect of Met inhibitor BMS-777607 on PC-3 cell functions PC-3 cells were treated with indicated doses of BMS-777607 for 4 days (A), 14 days (B), 1 day (C), 2 days (D, E) and 3 days (F), for the detection of cell growth (MTT assay, A), clonogenicity (colony formation assay, B), cell motility [“wound-healing” assay, C (magnification, ×5)], migration (transwell assay, D), invasion (transwell assay, E) and anoikis (trypan blue exclusion assay, F), respectively. Data either represent 1 of 3 independent experiments (B, C) or are from > 3 independent experiments (A, D, E, F). A, B, F: Columns, mean; bars, SD (n≥9). D, E: boxes, median (middle line) with 75th (upper boundary) and 25th (lower boundary) percentiles; whiskers, 90th (upper) and 10th (lower) percentiles (n = 8). *, P < 0.05 (t-test in A and Wilcoxon test in D, E); ***, P < 0.001 (t-test in A, B, F). Wort = Wortmannin.