Detection of HGF and c-Met in prostate cancer cells A, qPCR analysis of HGF and c-Met RNA levels in PC-3 and DU145 cells. Gene expression in PC-3 cells was defined as “1”. Columns, mean; bars, SD (n = 3). ND = not detectable. B, PC-3 cells were incubated in serum-free medium for 24 h. Condition medium (CM) was harvested and an aliquot amount (from 106 cells) was analyzed by Western blot under both non-reducing and reducing conditions, with the recombinant human HGF (10 ng/lane) as a positive control. MW = molecular weight. kDa = kilo Dalton. Data are from 1 of 2 independent experiments.