Figure 4

Effects on the motility and matrigel invasion of HEC-50B and KLE cells after transfection with the emmprin siRNA. A) Cell scratch assays of HEC-50B and KLE cells after transfection with the emmprin siRNA for 24 h. B) Migration assays on type IV collagen for HEC-50B and KLE cells after transfection with the emmprin siRNA for 72 h. C) Matrigel invasion assays after transfection of the emmprin siRNA into HEC-50B and KLE cells for 72 h. Following the incubation, the membranes were removed from the inserts and mounted on slides. The numbers of invading cells were counted under a microscope. The motility and Matrigel assays were performed in triplicate.