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Table 2 Promoter methylation status and expression levels of KDR and FLT4

From: DNA methylation regulates expression of VEGF-R2 (KDR) and VEGF-R3 (FLT4)

   KDR    FLT4  
  MSP mRNA protein MSP mRNA protein
697 M 0.08 neg M/U 0 neg
ALL-SIL M 0 neg U 0.3 neg
AP-1060 M/U 0 n.d. M/U 0 n.d.
BV-173 M 0 neg U 1.4 pos
CMK M/U 1 pos M 0.1 neg
DOHH-2 M/U 0 neg M/U 0 neg
EM-2 M/U 0 neg M 0 neg
HANK-1 M 0 neg M 0 neg
HEL U 2.6 pos U 1 pos
HL-60 M 0 neg M 0.1 neg
JURL-MK1 U 0.15 n.d. n.d. n.d. n.d.
L-82 M 0 n.d. M 0 n.d.
LOUCY n.d. 0.13 neg M/U 0 neg
M-07e M/U 0.03 neg U 0.1 neg
MEG-01 U 5.8 pos U 0.2 pos
MEGAL M 0 neg M/U 0.3 neg
MHH-CALL2 M/U 0 neg U 1.1 pos
MHH-TALL1 M 0.08 n.d. n.d. n.d. n.d.
MOLT-4 M 0 n.d. M/U 0 n.d.
MUTZ-3 n.d. n.d. n.d. U 0.6 neg
MUTZ-8 n.d. n.d. n.d. M/U 0.3 neg
NK-92 M 0 neg M/U 0 neg
OCI-AML1 M/U 0 neg M/U 1.3 pos
SC-1 M 0 n.d. M 0 n.d.
SKNO-1 U 0.08 neg n.d. n.d. n.d.
SUP-B15 M 0 neg U 1.6 pos
TF-1 U 0.1 pos U 0 neg
THP-1 M/U 0 n.d. M/U 0 n.d.
  1. KDR and FLT4 methylation was determined by MSP. M, positive in M-PCR; U, positive in U-PCR, M/U positive in M- and in U-PCR; n.d., not done. VEGF-R mRNA expression levels were determined by quantitative real-time PCR. Cell lines CMK (KDR) and HEL (FLT4) were used for normalization (set to 1). Protein expression was done by Western blot analysis. The accuracy of KDR M-PCR (mRNA positivity > 0.1) is 88%, the accuracy of FLT4 M-PCR (mRNA positivity > 0.1) is 80%