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Table 2 Promoter methylation status and expression levels of KDR and FLT4

From: DNA methylation regulates expression of VEGF-R2 (KDR) and VEGF-R3 (FLT4)

  

KDR

  

FLT4

 
 

MSP

mRNA

protein

MSP

mRNA

protein

697

M

0.08

neg

M/U

0

neg

ALL-SIL

M

0

neg

U

0.3

neg

AP-1060

M/U

0

n.d.

M/U

0

n.d.

BV-173

M

0

neg

U

1.4

pos

CMK

M/U

1

pos

M

0.1

neg

DOHH-2

M/U

0

neg

M/U

0

neg

EM-2

M/U

0

neg

M

0

neg

HANK-1

M

0

neg

M

0

neg

HEL

U

2.6

pos

U

1

pos

HL-60

M

0

neg

M

0.1

neg

JURL-MK1

U

0.15

n.d.

n.d.

n.d.

n.d.

L-82

M

0

n.d.

M

0

n.d.

LOUCY

n.d.

0.13

neg

M/U

0

neg

M-07e

M/U

0.03

neg

U

0.1

neg

MEG-01

U

5.8

pos

U

0.2

pos

MEGAL

M

0

neg

M/U

0.3

neg

MHH-CALL2

M/U

0

neg

U

1.1

pos

MHH-TALL1

M

0.08

n.d.

n.d.

n.d.

n.d.

MOLT-4

M

0

n.d.

M/U

0

n.d.

MUTZ-3

n.d.

n.d.

n.d.

U

0.6

neg

MUTZ-8

n.d.

n.d.

n.d.

M/U

0.3

neg

NK-92

M

0

neg

M/U

0

neg

OCI-AML1

M/U

0

neg

M/U

1.3

pos

SC-1

M

0

n.d.

M

0

n.d.

SKNO-1

U

0.08

neg

n.d.

n.d.

n.d.

SUP-B15

M

0

neg

U

1.6

pos

TF-1

U

0.1

pos

U

0

neg

THP-1

M/U

0

n.d.

M/U

0

n.d.

  1. KDR and FLT4 methylation was determined by MSP. M, positive in M-PCR; U, positive in U-PCR, M/U positive in M- and in U-PCR; n.d., not done. VEGF-R mRNA expression levels were determined by quantitative real-time PCR. Cell lines CMK (KDR) and HEL (FLT4) were used for normalization (set to 1). Protein expression was done by Western blot analysis. The accuracy of KDR M-PCR (mRNA positivity > 0.1) is 88%, the accuracy of FLT4 M-PCR (mRNA positivity > 0.1) is 80%