Bisulfite sequencing of the FLT4 promoter. A CpG island is located between -1231 and 769 relative to the ATG codon of FLT4. Part of the promoter region (337 bp, 20 CpG sites) was sequenced from cell lines EM-2 (FLT4 negative) and SUP-B15 (FLT4 positive) as well as from HDEMCs and HUVECs. Each line depicts a sequenced clone representing the methylation status of an individual allele. CpGs are represented as open dots (if unmethylated) or filled dots (if methylated). Results of qRT-PCR and methylation specific PCR (MSP) are shown on the left hand side. M: signal in M-PCR; U: signal in U-PCR.