(A) Chemical structures of Chrysin, Methoxy Chrysin and Oroxylin A. (B) Chrysin affects cell viability. Human melanoma A375 cells were seeded at density of 10,000 cells per well of 96 well plate and treated with 40 μM of Oroxylin A (Oro), Methoxy Chrysin (Meo Chry), Chrysin (Chry) at a concentration of 40 μM and 4 μM Trichostatin A (TSA) for 24 h. The cell viability of A375 cells was measured by MTT assay 24 h after compound treatment. Data represents mean ± S.D of three independent experiments performed. *** represents P < 0.001; ** represents p < 0.01. (C) Comparative studies between chrysin (40 and 120 μM) and TSA (2, 4 and 12 μM) was made and the cell viability was analysed.