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Table 1 Primers for PCR, real-time PCR and Oligonucleotides for Rab11a gene silence

From: Impaired degradation followed by enhanced recycling of epidermal growth factor receptor caused by hypo-phosphorylation of tyrosine 1045 in RBE cells

EGFR exon 18 ~ 21

Sense

cctaagatcccgtccatcgcc

 

Antisense

cactttgcctccttctgcatggta

EGFR exon 22 ~ 27

Sense

ttgggctggccaaactgctgg

 

Antisense

caggcactgggaggaaggtgt

EGFR exon 28

Sense

ccacaggcgccttgactgagga

 

Antisense

gcaacttcccaaaatgtgcccg

 

Sense No.1

agtcgggctctggaggaa

 

Antisense No.1

ggcagttctcctctccg

 

Sense No.2

ctgtgcaacgtggagagc

EGFR

Antisense No.2

ccatctcatagctgtcgg

exon 2 ~ 16 (5 pairs)

Sense No.3

cccaccacgtaccagatg

 

Antisense No.3

ccatgttgcttggtcctgcc

 

Sense No.4

gctgattcaggcttggcc

 

Antisense No.4

ctcaccctccagaagcttgc

 

Sense No.5

ggtctgccatgccttgtg

 

Antisense No.5

ggcccattcgttggacag

EGFR (real-time PCR)

Sense

gcacctacggatgcactgg

 

Antisense

ggcgatggacgggatctta

β-actin (real-time PCR)

Sense

acgtggacatccgcaaagac

 

Antisense

caagaaagggtgtaacgcaacta

Rab11a (real-time PCR)

Sense

ggcacagatatgggacacagc

 

Antisense

aaggcacctacagctccacg

Rab11a siRNA

Sense

aaugucagacagacgcgaaaatt

 

Antisense

uuuucgcgucugucugacauutt