Figure 4From: Circulating tumour cells escape from EpCAM-based detection due to epithelial-to-mesenchymal transition Quantitative detection of CTCs targeting human Alu-sequences. 1 to 100,000 human cancer cells were spiked into 200 μl of tumour-free murine blood. DNA was isolated and qRT-PCR was performed targeting human Alu-sequences. As little as 1 human cell could be detected before negative controls. Measurements were done in triplicates (n.d. = not defined).Back to article page