Inhibition of VEGFR-2 signaling by IDR-E804. (A) HUVECs were incubated with VEGF (10 ng/mL) and the indicated concentration of IDR-E804 for 30 min. Total cell lysates were prepared, proteins were separated by SDS–PAGE, and Western blot analysis was conducted. Photographs of chemiluminescent detection of the blots representative of four independent experiments are shown. Band intensity was quantified by densitometry and each bar represents the mean ± S.E. *
P < 0.05, **
P < 0.01, ***
P < 0.001 versus VEGF-treated HUVECs. (B) Inhibition of VEGFR-2 activation by IDR-E804 in a specific VEGFR-2 inhibition assay. The results are reported as the mean ± S.E. of triplicate assays.