Figure 2

The Cas III-ia induces autophagic marks. A) Analysis of LC-3 (red-rodamine and blue–DAPI) by confocal microscope (left panel) and Western blot of LC-3 (right panel). B) Beclin 1 and Atg7 expression in cell lysates of control and Cas III-ia-treated glioma C6 cells were determined by Western blot. C) Accumulation of LysoTracker-red was analyzed in 24 h Cas III-ia-treated glioma C6 cells and controls by confocal microscope. The figures shown are representative of at least three different experiments for each experimental condition.