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Figure 1 | BMC Cancer

Figure 1

From: Inhibitory effect of Bifidobacterium infantis-mediated sKDR prokaryotic expression system on angiogenesis and growth of Lewis lung cancer in mice

Figure 1

Results of electrophoresis and Western blot. (A) PCR products of sKDR. Lane 1: sKDR; Lane 2: DNA Marker III. (B) Endonuclease digestion and PCR products. Lane 1: DNA Ladder; Lane 2: digestion of recombinant plasmid pTRKH2-PsT/sKDR; Lane 3: recombinant plasmid pTRKH2-PsT/sKDR; Lane 4: plasmid pTRKH2-PsT; Lane 5: PCR products of sKDR gene from recombinant plasmid pTRKH2-PsT/sKDR; Lane 6: PCR products of sKDR gene from recombinant plasmid pcDNA3.1/sKDR; Lane 7: DNA Marker. (C) RT-PCR and PCR products from recombinant Bifidobacterium infantis of pTRKH2-PsT/sKDR. Lane 1: PCR products of sKDR gene from recombinant plasmid pTRKH2-PsT/sKDR; Lane 2: RT-PCR product of RNA purified from Bifidobacterium infantis with pTRKH2-PsT/sKDR; Lane 3: DNA Marker. (D) Western blot analysis of sKDR expression. Lane 1: pTRKH2-PsT/sKDR; Lane 2: pTRKH2-PsT.

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