PAX3-FKHR binds to the CPT1A promoter. (A) EMSA analysis of GFP–PAX3-FKHR binding to CPT1A putative binding site 2 (PRO2). All lanes contain labeled PRO2 in addition to other components, as indicated. 100-fold excess unlabeled wild-type (PRO2) or PD-binding site mutant (PRO2m) was used to compete with the labeled PRO2 DNA probe for GFP-PAX3-FKHR binding. GFP-PF, nuclear extract prepared from HEK293T cells transfected with GFP-PAX3-FKHR; GFP, nuclear extract prepared from HEK293T cells transfected with GFP vector only. Arrow indicates the PAX-FKHR-DNA complexes. (B ) The amount of GFP-PF and GFP in the nuclear extracts used for the EMSA, revealed by using an anti-GFP antibody in a Western blot analysis.