Figure 3
PAX3-FKHR regulates the expression of CPT1A. Levels of CPT1A and PAX3-FKHR mRNA were determined by using quantitative real time RT-PCR or Western blot. (A) The mRNA levels of PAX3-FKHR or CPT1A in control Rh30 cells was arbitrarily set as 1, and the mRNA levels of PAX3-FKHR or CPT1A measured under other experimental conditions were expressed as “mRNA fold change” by normalizing them to the corresponding mRNA level in Rh30 cells. Rh30_PF siRNA, Rh30 cells transiently transfected with PF siRNA; Rh41_PF siRNA, Rh41 transiently transfected with PF siRNA. Rh30_NT siRNA, Rh41_NT siRNA, and RD represents Rh30, Rh41, and RD cells transiently transfected with NT siRNA, respectively. (B) Protein levels of PAX3-FKHR (GFP-PF and PF) and CPT1A in cells (NIH3T3 or RD) transiently transfected with either GFP or GFP-PF, or Rh30 transiently transfected with either NT siRNA or PF siRNA. Actin was an equal loading control. (C) RD or NIH3T3 cells were transiently transfected with either GFP empty vector or GFP-PAX3-FKHR (GFP-PF). The mRNA levels of CPT1A in 3T3/GFP or RD/GFP was arbitrarily set as 1.