Figure 3
Tet-On system-controlled sTRAIL expression suppresses tumor cell growth by apoptosis. MDA-MB-231 and U251 cells were transduced for 48 h with rAAV-TRE-TRAIL&AAV-Tet-On at 2 × 105 GPs per cell. rAAV-TRE- EGFP&AAV-Tet-On and rAAV-CAG-TRAIL were used as negative and positive control, respectively. (A) The sTRAIL expression was detected by RT-PCR and Western blot assay. (B) The viability of tumor cells was evaluated by microscopy and flow cytometry. Dox+, 1 μg/ml doxycycline in the medium. Dox-, without doxycycline in the medium. WPRE, woodchuck hepatitis virus posttranscriptional regulatory element, represents sTRAIL mRNA expression. NTC: No-Template Control. PTC: Positive Template Control.