Skip to main content
Figure 5 | BMC Cancer

Figure 5

From: Multicellular tumor spheroid model to evaluate spatio-temporal dynamics effect of chemotherapeutics: application to the gemcitabine/CHK1 inhibitor combination in pancreatic cancer

Figure 5

Gemcitabine and CHIR-124 produce a synergistic cytotoxic effect on Capan-2 spheroid. Spheroids were treated for 72 h after 4 day initial culture with gemcitabine, CHIR-124 or a combination of the 2 compounds at concentration corresponding to their respective EC20 values. Spheroid viability was quantified at day 7 by ATP monitoring with the luminescent ATPlite assay. ATP content percentage was calculated with regard to non-treated spheroid and showed cell growth inhibition and/or toxicity.

Back to article page

BMC Cancer

ISSN: 1471-2407

Contact us