Distribution of proliferative and apoptotic cells in Capan-2 spheroids of different sizes. Ki-67 and cleaved form of PARP were analyzed by immunodetection on 5 μm frozen sections in Capan-2 spheroids of different size. 400, 600 and 800 μm correspond respectively to spheroid at days 4, 7, and 12 after culture initiation in defined medium supplemented with EGF and B27. The scale bar corresponds to 100 μm. Results shown are representative of the examination of 3 sections from 5 spheroids. Each experiment has been repeated 3 times.