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Figure 3 | BMC Cancer

Figure 3

From: Tetrathiomolybdate sensitizes ovarian cancer cells to anticancer drugs doxorubicin, fenretinide, 5-fluorouracil and mitomycin C

Figure 3

Activation of pro-apoptotic JNK and p38 MAPK after TM/doxorubicin treatment with or without inhibition of ROS. (a) SKOV-3 cells (left panel) were treated with TM (0, 30 μM) for 24 h, after which the cells were treated with doxorubicin (0, 5 μM) for 1, 4, 7 or 24 h as indicated. The same procedure was employed to treat A2780 cells (right panel) with different concentrations of TM (0, 7.5 μM) and doxorubicin (0, 2.5 μM). Immunoblotting was carried out with primary antibodies against native and activated/phosphorylated JNK or p38 MAPK. As an internal standard for equal loading blots were probed with an anti-GAPDH antibody. (b) SKOV-3 cells were treated as described in (a) in the presence or absence of radical scavenger ascorbic acid (750 μM). Immunoblotting was carried out with primary antibodies against JNK, p-JNK, p38, p-p38, cleaved PARP or GAPDH.

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