Figure 3

Whole genome expression profiling of prostate cancer cells treated with genistein, vorinostat, or the combination. (A) Hierarchical clustering of 1077 probes strongly affected by treatment with genistein and vorinostat. (B) Ingenuity Pathway Analysis (IPA) Network of genes annotated for function in Apoptosis. Red indicates increased expression after treatment, and green indicates reduced expression. Solid lines represent direct interactions and dashed lines represent indirect interactions. (C) IPA Network of genes annotated for function in DNA Repair and Cell Cycle. (D) QPCR data from a subset of genes with functions in apoptosis pathways normalized to ARCAP-E control cells. (E) QPCR confirmation of down regulation of TGFB1I1/ARA55, SLUG, BIRC7/Livin, and HES1 following genistein treatment. Data are presented as mean ± SE of triplicate experiments.