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Figure 9 | BMC Cancer

Figure 9

From: Therapeutic implications of an enriched cancer stem-like cell population in a human osteosarcoma cell line

Figure 9

Cell cycle and apoptotic responses of MNNG/HOS and CSCs to irradiation. Representative cell cycle phase distribution (A) and Hoescht-33342 nuclear staining (B) of MNNG/HOS and CSCs after irradiation at indicated doses. Forty-eight hours after irradiation, cells were fixed and stained with propidium iodide (PI) for cell cycle analysis by flow cytometry and with the DNA Hoescht-33342 dye to detect apoptosis. Irradiation induced a dose-dependent cell cycle arrest at G2/M phase and apoptosis in MNNG/HOS cells and had no significant effects on cell cycle progression in CSCs with minimal induction of apoptosis. Fluorescence images were acquired using a fluorescence microscope with an original magnification of × 630.

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