Figure 8

Cell cycle and apoptotic responses of MNNG/HOS and CSCs to chemotherapeutic agents. Representative cell cycle phase distribution and Hoescht-33342 nuclear staining of MNNG/HOS and CSCs after treatment with doxorubicin (A, D), cisplatin (B, E) and methotrexate (C, F). Cells were incubated with drugs at the indicated concentrations. After 48 h, cells were fixed and stained with propidium iodide for cell cycle analysis by FACS and with the DNA Hoescht-33342 dye to detect apoptosis. All drugs induced a cell cycle arrest and chromatin condensation in MNNG/HOS cells. In contrast, there were no significant changes in cell cycle distribution of CSCs and a few apoptotic cells were detected for the high drug concentrations. Fluorescence images were acquired using a fluorescence microscope with an original Magnification of × 630.