Figure 4
NO does not influence hormone-induced nuclear translocation of the AR. Prostate cancer cells (PC-3) expressing a green fluorescent AR-EosFP [37] were incubated for 6 hours in the absence/presence of DHT and JS-K/JS-Kneg. Intracellular localization of AR-EosFP was determined by fluorescence microscopy: (A) untreated controls, (B) 5 nM DHT, (C) 5 nM DHT and 4 μM JS-K, (D) 5 nM DHT and 4 μM JS-Kneg. Bars: Data presented in % cellular localization ± SD. Results are mean values of three independent experiments performed in quadruplicates.