|
vp
|
Primers
|
Sequence (5'-3')
|
Product size
|
Annealing Temp.(°C)
|
Cycles
|
|---|
|
RT-PCR
|
BZLF1F
|
GGGAGAAGCACCTCAACCTG
|
255-bp
|
55
|
35
|
| |
BZLF1R
|
TTGCTTAAACTTGGCCCGGC
| | | |
| |
BRLF1F
|
CAAACAGACGCAGATGAGGC
|
443-bp
|
55
|
35
|
| |
BRLF1R
|
GCGGTGCCTATGGTGGCAGG
| | | |
| |
BHRF1H2
|
GTCAAGGTTTCGTCTGTGTG
|
213-bp
|
55
|
35
|
| |
BHRF1H3
|
TTCTCTTGCTGCTAGCTCCA
| | | |
| |
BLLF1F
|
GTGGATGTGGAACTGTTTCCAG
|
230-bp
|
55
|
35
|
| |
BLLF1R
|
CTGTATCCACCGCGGATGTCAC
| | | |
| |
BARTA3
|
AGAGACCAGGCTGCTAAACA
|
232-bp
|
55
|
35
|
| |
BARTA4
|
AACCAGCTTTCCTTTCCGAG
| | | |
| |
GAPDHF
|
GATGACCTTGCCCACAGCCT
|
303-bp
|
55
|
23
|
| |
GAPDHR
|
ATCTCTGCCCCCTCTGCTGA
| | | |
|
MSP
|
Zpm1
|
TCGGATTTTCGTGGGTTAATC
|
101-bp
|
64
|
42
|
| |
Zpm2
|
CGAAACTAACGTTAAAAATCCG
| | | |
| |
Zpu1
|
ATTTGGATTTTTGTGGGTTAATT
|
101-bp
|
62
|
40
|
| |
Zpu2
|
CCAAAACTAACATTAAAAATCCA
| | | |
| |
Rpm1
|
TAGGTATTTTGGATATTCGTAC
|
172-bp
|
64
|
42
|
| |
Rpm3
|
GATTTAATCCCAACCGCCG
| | | |
| |
Rpu1
|
TGTAGGTATTTTGGATATTTGTAT
|
78-bp
|
62
|
40
|
| |
Rpu2
|
CAAATCATTACTCCAAACTATCA
| | | |
|
BGS
|
ZpMZP1
|
AAAAAACACCTAATATAAATCAAA
|
814-bp
|
62
|
42
|
| |
ZpSC22
|
GTTAAAGAGGATTAGGTTTTTTT
| | | |
| |
RpMRP1
|
TCTTTTATAAACCATTAACATAAA
|
599-bp
|
60
|
42
|
| |
RpMRP2
|
GAGATTGATAGGATTATATTGT
| | | |
