A mouse model for triple-negative breast cancer tumor-initiating cells (TNBC-TICs) exhibits similar aggressive phenotype to the human disease

Table 1 TNBC-TICs exhibit a greater clonogenic growth potential as compared to TNBC, TPBC-TICs, TPBC or parental 4T1 cells

Cell type¹	Number of cells transplanted	Fraction of mice with tumors²
TPBC-derived:
CD24⁺/ALDH1⁺/CD44^high	500	5/5
CD24⁺/ALDH1⁺/CD44^high	100	5/5
CD24⁺/ALDH1⁺/CD44^high	50	4/5
CD24⁺/ALDH1⁺/CD44^high	25	2/5
CD24⁺/ALDH1⁺/CD44^high	10	3/5
TPBC-derived:
CD24⁺/ALDH1^-/CD44^low	500	1/5
CD24⁺/ALDH1^-/CD44^low	100	0/5
CD24⁺/ALDH1^-/CD44^low	50	0/5
CD24⁺/ALDH1^-/CD44^low	25	0/5
CD24⁺/ALDH1^-/CD44^low	10	0/5
TNBC-derived:
CD24⁺/ALDH1⁺/CD44^high	500	5/5
CD24⁺/ALDH1⁺/CD44^high	100	5/5
CD24⁺/ALDH1⁺/CD44^high	50	5/5
CD24⁺/ALDH1⁺/CD44^high	25	5/5
CD24⁺/ALDH1⁺/CD44^high	10	4/5
TNBC-derived:
CD24⁺/ALDH1^-/CD44^low	500	1/5
CD24⁺/ALDH1^-/CD44^low	100	0/5
CD24⁺/ALDH1^-/CD44^low	50	0/5
CD24⁺/ALDH1^-/CD44^low	25	0/5
CD24⁺/ALDH1^-/CD44^low	10	0/5

¹Tumor cells derived from TPBC or TNBC cells were stably transfected with eGFP using the lentivirus transduction technique as described in detail in the Methods section
²Twenty-one days post tumor cell transplantation into the mammary pad of naïve female BALB/c mice, animals were scarified and eGFP signal from the tumors were measured using the Maestro™ in vivo imaging system (CRI), and spectral unmixing was performed to segregate skin and hair auto fluorescence and to measure the true eGFP signal, as described in detail in the Methods section. Data is the fraction of mice with tumors (n = 5)

ISSN: 1471-2407