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Table 1 TNBC-TICs exhibit a greater clonogenic growth potential as compared to TNBC, TPBC-TICs, TPBC or parental 4T1 cells

From: A mouse model for triple-negative breast cancer tumor-initiating cells (TNBC-TICs) exhibits similar aggressive phenotype to the human disease

Cell type1 Number of cells transplanted Fraction of mice with tumors2
TPBC-derived:   
CD24+/ALDH1+/CD44high 500 5/5
CD24+/ALDH1+/CD44high 100 5/5
CD24+/ALDH1+/CD44high 50 4/5
CD24+/ALDH1+/CD44high 25 2/5
CD24+/ALDH1+/CD44high 10 3/5
TPBC-derived:   
CD24+/ALDH1-/CD44low 500 1/5
CD24+/ALDH1-/CD44low 100 0/5
CD24+/ALDH1-/CD44low 50 0/5
CD24+/ALDH1-/CD44low 25 0/5
CD24+/ALDH1-/CD44low 10 0/5
TNBC-derived:   
CD24+/ALDH1+/CD44high 500 5/5
CD24+/ALDH1+/CD44high 100 5/5
CD24+/ALDH1+/CD44high 50 5/5
CD24+/ALDH1+/CD44high 25 5/5
CD24+/ALDH1+/CD44high 10 4/5
TNBC-derived:   
CD24+/ALDH1-/CD44low 500 1/5
CD24+/ALDH1-/CD44low 100 0/5
CD24+/ALDH1-/CD44low 50 0/5
CD24+/ALDH1-/CD44low 25 0/5
CD24+/ALDH1-/CD44low 10 0/5
  1. 1Tumor cells derived from TPBC or TNBC cells were stably transfected with eGFP using the lentivirus transduction technique as described in detail in the Methods section
  2. 2Twenty-one days post tumor cell transplantation into the mammary pad of naïve female BALB/c mice, animals were scarified and eGFP signal from the tumors were measured using the Maestro™ in vivo imaging system (CRI), and spectral unmixing was performed to segregate skin and hair auto fluorescence and to measure the true eGFP signal, as described in detail in the Methods section. Data is the fraction of mice with tumors (n = 5)