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Table 2 Results of internal controls of conversion of the three markers

From: Why do results conflict regarding the prognostic value of the methylation status in colon cancers? the role of the preservation method

Identity FFPE DNA 1 FFPE DNA 2 FFPE DNA 2 FFPE DNA 2 Cryo. DNA Cryo. DNA Cryo. DNA
  LINE-1 LINE-1 MLH1 MGMT LINE-1 MLH1 MGMT
  PCR 1 PCR 2 PCR 1 PCR 2 PCR 1 PCR 2 PCR 1 PCR 2 PCR 1 PCR 2 PCR 1 PCR 2 PCR 1 PCR 2
1 check failed passed passed passed   passed   passed passed passed   passed  
2 failed passed check passed   passed   passed passed passed   passed  
3 passed passed check check   failed failed passed passed passed   passed  
4 failed failed passed check   failed passed passed passed passed   passed  
5 failed passed failed check passed passed passed passed passed passed   passed  
6 check passed passed passed passed   passed   passed passed passed   passed  
7 failed failed failed check passed passed failed passed passed passed passed   check check
8 passed failed passed passed passed   passed   passed passed passed   passed  
9 check passed passed passed passed   passed   passed passed passed   passed  
10 failed failed passed passed passed   passed   passed passed passed   passed  
11 failed failed check passed passed check check passed passed passed   passed  
12 failed failed failed check passed passed check passed passed passed   passed  
13 check check passed passed passed   passed   passed passed passed   passed  
14 check passed passed passed passed passed   passed passed passed   passed  
15 check check passed passed passed   passed   passed passed passed   passed  
16 passed passed check check passed passed passed passed passed passed   passed  
17 failed failed passed passed passed   check passed passed passed passed   passed  
18 passed failed passed passed   passed   passed passed passed   passed  
19 check failed failed passed   check passed check passed passed   failed check
20 passed check passed passed passed   failed passed passed passed passed   passed  
21 check check failed check   failed failed passed passed check failed passed  
22 passed check check check passed   failed passed passed passed passed   passed  
23 passed check passed passed passed   passed   passed passed passed   passed  
24 passed passed passed passed passed   passed   passed passed passed   passed  
25 passed failed passed passed   passed passed passed passed passed   passed  
26 check check passed passed passed   check passed passed passed passed   check check
27 check passed passed passed passed   passed   failed    
28 passed passed passed passed passed   failed failed passed passed passed   passed  
29 check check passed passed passed   passed   passed passed passed   passed  
30 passed passed passed passed failed check check passed passed passed passed   passed  
31 failed failed passed passed   passed   passed passed passed   passed  
32 passed passed passed passed passed   passed   passed passed passed   passed  
33 passed passed check check passed check passed passed passed passed   passed  
34 check check passed passed   failed passed passed passed passed   passed  
35 check failed passed passed   failed passed passed passed passed   passed  
36 passed passed passed passed failed failed passed   passed passed passed   passed  
37 check passed check failed passed passed failed failed passed passed passed   check check
38 passed passed check check passed passed passed   passed passed passed   failed failed
39 check check passed check passed   check passed passed passed passed   passed  
40 check check passed passed   failed passed passed passed passed   passed  
  1. From left to right the table gives results for DNA from FFPE tissues extracted with the classical method (FFPE DNA 1), extracted with the dedicated method (FFPE DNA 2) and DNA from cryo-preserved tissues (Cryo. DNA). "Passed" indicates a good conversion (0.0 to 5.0% of unconverted cytosines). "Check" indicates an uncertain conversion (5.0 to 7.0% of unconverted cytosines). "Failed" indicates a poor conversion (more than 7.0% of unconverted cytosines). Slash bar indicates there was no amplification. Results in bold emphasize controls of conversion giving "check" or "failed" results.